Recombinant DNA Technology

Question: Which of the following techniques can be used to determine the defective gene and for developing cancer?
[A].

Western blot

[B].

Southern blot

[C].

Northern blot

[D].

Eastern blot

Answer: Option B

Explanation:

No answer description available for this question.

Question: X-rays cause
[A].

the formation of thymine dimers

[B].

ionization of water in the cell

[C].

heat

[D].

none of the above

Answer: Option B

Explanation:

No answer description available for this question.

Question: Which of the following pair will produce complementary sticky ends?
[A].

Eco RI & MspI

[B].

Msp I & HPA II

[C].

Sau 3A & Bam HI

[D].

Mbo I & Sau 3A

Answer: Option C

Explanation:

No answer description available for this question.

Question: Isoschizomers recognize
[A].

same recognition sequence but different recognition site

[B].

same recognition site and recognition sequence

[C].

same recognition site and different recognition sequence

[D].

different recognition site and different recognition sequence

Answer: Option B

Explanation:

No answer description available for this question.

Question: First discovered, Type II restriction endonuclease was
[A].

Hinf I

[B].

Eco K

[C].

Hind II

[D].

EcoRI

Answer: Option C

Explanation:

No answer description available for this question.

Question: Which type of restriction enzymes do not usually require ATP?
[A].

Type I

[B].

Type II

[C].

Type III

[D].

Type IV

Answer: Option B

Explanation:

No answer description available for this question.

Question: Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because
[A].

in both cases molecules migrate to the anode

[B].

both techniques rely on a constant charge to mass ratio

[C].

both techniques utilize the sieving properties of gels

[D].

all of the above

Answer: Option D

Explanation:

No answer description available for this question.

Question: A human cDNA library
[A].

contains DNA for specific human proteins

[B].

contains DNA for virtually all of the human proteins in vectors

[C].

cannot be used to obtain human genes because it would be radioactive

[D].

none of the above

Answer: Option B

Explanation:

No answer description available for this question.

Question: An expression vector
[A].

always contains an origin of replication

[B].

usually contains a gene that confers antibiotic resistance to the bacterial host

[C].

always contains DNA segments for the regulation of mRNA production

[D].

all of the above

Answer: Option D

Explanation:

No answer description available for this question.

Question: A plasmid
[A].

is a circular DNA molecule

[B].

always contains an origin of replication

[C].

usually contains one or more restriction sites

[D].

all of the above

Answer: Option D

Explanation:

No answer description available for this question.