Recombinant DNA

Question: Agrobacterium tumefaciens is
[A].

a disease in humans that causes loss of sight

[B].

a bacterium that can be used to introduce DNA into plants

[C].

a fungi that is used to produce antibiotics in large amounts

[D].

a disease in humans that causes loss of weight

Answer: Option B

Explanation:

No answer description available for this question.

Question: Electroporation is
[A].

the process of separating charged molecules through a gel maintained in an electric field

[B].

the process of combining foreign DNA to an electrically charged vector molecule

[C].

the application of high voltage pulses

[D].

the process of multiplication of the cells

Answer: Option C

Explanation:

No answer description available for this question.

Question: An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as
[A].

a chimera

[B].

a transgenic animal

[C].

a vector

[D].

an enzyme that links DNA molecules

Answer: Option B

Explanation:

No answer description available for this question.

Question: Which of the following vector can maintain the largest fragment of foreign DNA?
[A].

YAC

[B].

Cosmid

[C].

Plasmid

[D].

Phage

Answer: Option A

Explanation:

No answer description available for this question.

Question: In genetic engineering, a chimera is
[A].

an enzyme that links DNA molecules

[B].

a plasmid that contains foreign DNA

[C].

a virus that infects bacteria

[D].

a fungi

Answer: Option B

Explanation:

No answer description available for this question.

Question: For gene probes to be useful they must
[A].

be large enough to contain gene-specific sequences

[B].

be labeled in some manner to allow detection

[C].

both (a) and (b)

[D].

none of the above

Answer: Option C

Explanation:

No answer description available for this question.

Question: A genomic library is
[A].

a database where the sequence of an organism’s genome is stored

[B].

a collection of many clones possessing different DNA fragments from the same organisms bound to vectors

[C].

a book that describes how to isolate DNA from a particular organism

[D].

a place where the information of the genetic organization of organisms are kept.

Answer: Option B

Explanation:

No answer description available for this question.

Question: The advantage of using DNA polymerases from thermophilic organisms in PCR is that
[A].

the DNA polymerases of these bacteria are much faster than those from other organisms

[B].

the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

[C].

the DNA polymerases of these bacteria never make mistakes while replicating DNA

[D].

all of the above

Answer: Option B

Explanation:

No answer description available for this question.

Question: A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as
[A].

southern blotting

[B].

northern blotting

[C].

polymerase chain reaction

[D].

DNA replication

Answer: Option C

Explanation:

No answer description available for this question.

Question: A short molecule containing 2-20 nucleotide is
[A].

plasmid

[B].

vector

[C].

oligonucleotide

[D].

mononucleotide

Answer: Option C

Explanation:

No answer description available for this question.