Immunological Techniques

Question: Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?
[A].

Open circular, super coiled, linear plasmid DNA

[B].

Linear plasmid, open circular, super coiled plasmid

[C].

Open circular, super coiled, linear plasmid DNA

[D].

Open circular, linear plasmid, super coiled plasmid

Answer: Option B

Explanation:

No answer description available for this question.

Question: Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively
[A].

2000 kb and 20kb DNA

[B].

1000 kb and l0kb DNA

[C].

20 kb and 2000 kb DNA

[D].

10 kb and 1000 kb DNA

Answer: Option C

Explanation:

No answer description available for this question.

Question: Agarose a porous matrix used in various molecular biology technique is made by repetitive units of
[A].

maltose

[B].

agarobiose

[C].

agar

[D].

none of these

Answer: Option A

Explanation:

No answer description available for this question.

Question: DNA- footprinting is a suitable technique for identifying which of the following?
[A].

Particular mRNA in mixture

[B].

Particular t-RNA in mixture

[C].

Introns within DNA

[D].

Protein binding site within DNA

Answer: Option D

Explanation:

No answer description available for this question.

Question: In Counter immunoelectrophoresis
[A].

electrophoresis will drive the antibody and antigen parallel to each other

[B].

electrophoresis will drive the antibody and antigen toward each other

[C].

the antibody will migrate towards anode

[D].

the antibody will migrate towards cathode

Answer: Option B

Explanation:

No answer description available for this question.

Question: In Rocket Immunodiffusion the length of the rocket is
[A].

proportional to the amount of antibody placed in each well

[B].

inversely proportional to the amount of antibody placed in each well

[C].

inversely proportional to the amount of antigen placed in each well

[D].

proportional to the amount of antigen placed in each well

Answer: Option D

Explanation:

No answer description available for this question.

Question: Which of the following radioactive element is generally involved in RIA?
[A].

Tritium

[B].

Carbon-14

[C].

Iodine-125

[D].

All of these

Answer: Option D

Explanation:

No answer description available for this question.

Question: An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that
[A].

there was more than one antigen

[B].

the antibody was impure

[C].

the temperature was too high

[D].

there was one antibody and one antigen

Answer: Option A

Explanation:

No answer description available for this question.