Animal Cell Culture and Regulation

Question: During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture?
[A].

Ethyl alcohol is being produced in excess

[B].

The cells have too much oxygen

[C].

Glycolysis is being inhibited

[D].

The cells do not have enough oxygen

Answer: Option D

Explanation:

No answer description available for this question.

Question: Disaggregating of cells can be achieved by
[A].

physical disruption

[B].

enzymatic digestion

[C].

treating with chelating agents

[D].

all of the above

Answer: Option D

Explanation:

No answer description available for this question.

Question: In animal cell culture, particularly mammalian cell culture, transformation means
[A].

uptake of new genetic material

[B].

phenotypic modifications of cells in culture

[C].

both (a) and (b)

[D].

release of genetic information

Answer: Option B

Explanation:

No answer description available for this question.

Question: Cells which have undergone transformation frequently become
[A].

anchorage independent

[B].

anchorage dependent

[C].

stable

[D].

unstable

Answer: Option A

Explanation:

No answer description available for this question.

Question: According to Eagle, the growth of L-strain and Hela-strain cultures require to have mandatory presence of
[A].

6 amino acids

[B].

8 amino acids

[C].

10 amino acids

[D].

12 amino acids

Answer: Option D

Explanation:

No answer description available for this question.

Question: For culturing, plasma from the adult chicken is preferred to mammalian plasma because
[A].

it forms a clear and solid coagulum even after dilution

[B].

it is too opaque

[C].

it doesn’t produce solid clots

[D].

it forms a semi solid coagulum

Answer: Option A

Explanation:

No answer description available for this question.

Question: An established cell line can be called where it has been subcultured at least
[A].

70 times at an interval of 3 days between subcultures

[B].

40 times at an interval of 3 days between subcultures

[C].

70 times at an interval of 1 day between subcultures

[D].

50 times at an interval of 3 days between subcultures

Answer: Option A

Explanation:

No answer description available for this question.

Question: At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via
[A].

oxidative pathway

[B].

anaerobic pathway

[C].

both (a) and (b)

[D].

none of these

Answer: Option A

Explanation:

No answer description available for this question.

Question: Accumulation of lactate leads to
[A].

increase in pH

[B].

no change in pH

[C].

reduction in the pH of culture hence loss of cell viability

[D].

no loss of cell viability

Answer: Option C

Explanation:

No answer description available for this question.

Question: Excess CO2 suppress cell growth and productivity by
[A].

inhibiting respiration

[B].

altering intracellular pH by diffusing across cell membrane

[C].

both (a) and (b)

[D].

altering pH of the medium

Answer: Option C

Explanation:

No answer description available for this question.