Vectors Uses For Animal Cell Culture

Question: Which of the following promoters is repressed in presence of glucose?
[A].

Galactokinase (GAL 1)

[B].

Acid phosphatase (PHOS)

[C].

Both (a) and (b)

[D].

Phosphoglycerate kinase (PGK)

Answer: Option A

Explanation:

No answer description available for this question.

Question: Cloning genes in the yeast offer which of following advantage(s)?
[A].

Over production of proteins of commercial value

[B].

Ability to glycosylate proteins during secretion

[C].

Ability to clone large piece of DNA

[D].

All of the above

Answer: Option D

Explanation:

No answer description available for this question.

Question: Autonomously replicating sequence is not present in
[A].

YIp

[B].

YRp

[C].

YCp

[D].

YAC

Answer: Option A

Explanation:

No answer description available for this question.

Question: YAC vectors can be used to obtain artificial chromosomes because it carries
[A].

centromere

[B].

telomere

[C].

autonomously replicating sequence

[D].

all of these

Answer: Option D

Explanation:

No answer description available for this question.

Question: Yeast Replicating plasmids (YRp) carry
[A].

centromere

[B].

telomere

[C].

autonomously replicating sequence

[D].

both (a) and (c)

Answer: Option C

Explanation:

No answer description available for this question.

Question: Yeast episomal plasmids (YEp) were constructed using
[A].

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9

[B].

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

[C].

12μm yeast plasmid and E coli vector pMB 9

[D].

12μm yeast plasmid and E coli vector pBR 322

Answer: Option A

Explanation:

No answer description available for this question.

Question: α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is
[A].

His 3

[B].

Lys 2

[C].

Ura 3

[D].

Trp 1

Answer: Option B

Explanation:

No answer description available for this question.

Question: Which of the following(s) is/are the most commonly used markers in yeast?
[A].

His 3

[B].

Ura 3

[C].

Trp 1

[D].

All of these

Answer: Option D

Explanation:

No answer description available for this question.

Question: Maximum insert size in case of adenovirus vectors is
[A].

6-8 kb

[B].

4-6 kb

[C].

10-12 kb

[D].

20 kb

Answer: Option A

Explanation:

No answer description available for this question.

Question: Moloney-Mu-leukemia virus is an example of
[A].

Vaccinia virus

[B].

Baculo virus

[C].

Retro virus

[D].

none of these

Answer: Option C

Explanation:

No answer description available for this question.