Immobilized Enzyme

Question: Various techniques are available for determining the effective diffusivity of solute in gel. Thin disk method uses
[A].

a diffusion cell with two compartments divided by a thin gel

[B].

a diffusion cell with two compartments

[C].

a diffusion cell with two compartments plus by a thin gel

[D].

a diffusion cell with two compartments multiply by a thin gel

Answer: Option A

Explanation:

No answer description available for this question.

Question: The effectiveness factor increases with the
[A].

increase of diffusivity and decreased with the increase of particle size

[B].

increase of diffusivity and increased with the increase of particle size

[C].

decrease of diffusivity and decreased with the increase of particle size

[D].

increase of diffusivity and decrease with the decrease of particle size

Answer: Option A

Explanation:

No answer description available for this question.

Question: For a steady state condition, the change of substrate concentration (dCs/dt) is
[A].

zero

[B].

1

[C].

>1

[D].

<1

Answer: Option A

Explanation:

No answer description available for this question.

Question: Which of the following is taken as an assumption in the distributed model?
[A].

The reaction occurs at every position and the kinetics of the reaction are of the same form as observed for free enzyme.

[B].

Mass transfer through the immobilized enzyme occurs via molecular diffusion

[C].

There is no mass transfer limitation at the outside surface of the spherical immobilized enzyme

[D].

All of the above

Answer: Option D

Explanation:

No answer description available for this question.

Question: To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, η can be defined as
[A].

reaction rate/rate if not slowed by diffusion

[B].

rate if not slowed by diffusion/ reaction rate

[C].

actual reaction rate/ rate if not slowed by diffusion

[D].

rate if not slowed by diffusion/ actual reaction rate

Answer: Option C

Explanation:

No answer description available for this question.

Question: If Damkohler number, NDa >>1, the reaction rate is much greater than the mass transfer rate and the overall reaction is controlled by the rate of mass transfer. In this case, the enzyme reaction can be described as (where Csb and Cs are the substrate concentration in bulk of solution and at the immobilized enzyme surface respectively. ks mass transfer coefficient, and a is the surface area of an immobilized enzyme particles)
[A].

rp = ksa(Csb-Cs)

[B].

rp = ksaCsb

[C].

rp = ksaCs

[D].

rp = ksa(Csb+Cs)

Answer: Option B

Explanation:

No answer description available for this question.

Question: For glucose isomerization by immobilized enzyme, the reactor generally used is
[A].

CSTR

[B].

plug flow

[C].

packed bed

[D].

fluidized bed

Answer: Option C

Explanation:

No answer description available for this question.

Question: Which medium is used for the production of Penicillin using immobilized cells
[A].

1% peptone medium

[B].

glucose medium

[C].

Yeast extract medium

[D].

LB broth

Answer: Option B

Explanation:

No answer description available for this question.

Question: The support material for immobilization of cells of Bacillus subtilis is
[A].

ion exchange resins

[B].

gelatin

[C].

Anthracite

[D].

agarose and carbodiimide

Answer: Option D

Explanation:

No answer description available for this question.

Question: During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is
[A].

equal to that of substrate consumption

[B].

more than that of substrate consumption

[C].

lesser than that of substrate consumption

[D].

is nothing to do with the substrate consumption

Answer: Option A

Explanation:

No answer description available for this question.