Gene Transfer In Plants

Question: Co-integrating transformation vectors must include a region of homology in
[A].

the vector plasmid

[B].

the Ti-plasmid

[C].

between vector plasmid and Ti-plasmid

[D].

none of these

Answer: Option C

Explanation:

No answer description available for this question.

Question: In the liposome mediated gene transfer in plants, nucleic acids are
[A].

protected from nuclease digestion

[B].

stable in liposomes

[C].

both (a) and (b)

[D].

not stable in liposomes

Answer: Option C

Explanation:

No answer description available for this question.

Question: Liposomes mediated gene transfer in plants involves
[A].

plasmid DNA enclosed in a lipid bag

[B].

fusion of liposomes with protoplast

[C].

use of polyethylene glycol (PEG)

[D].

all of the above

Answer: Option D

Explanation:

No answer description available for this question.

Question: Advantage of microprojectile method over microinjection method for gene transfer in plants include
[A].

intact cells are used

[B].

method is universal in its application irrespective of all shape, size, type and presence or absence of cell wall

[C].

gene can be transferred to many cells simultaneously

[D].

all of the above

Answer: Option D

Explanation:

No answer description available for this question.

Question: Microprojectile method of gene transfer in plants involves delivery of DNA
[A].

with the help of micromanipulator

[B].

with the help of bolistics

[C].

with the help of needles

[D].

any of the above

Answer: Option B

Explanation:

No answer description available for this question.

Question: Microinjection involves
[A].

injection of large amount of DNA

[B].

injection of DNA into bigger cells

[C].

injection with needle having diameter greater than cell diameter

[D].

all of the above

Answer: Option C

Explanation:

No answer description available for this question.

Question: Direct DNA uptake by protoplasts can be stimulated by
[A].

polyethylene glycol (PEG)

[B].

decanal

[C].

luciferin

[D].

all of these

Answer: Option A

Explanation:

No answer description available for this question.

Question: Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?
[A].

pRK 2013

[B].

pRN 3

[C].

either (a) or (b)

[D].

none of these

Answer: Option C

Explanation:

No answer description available for this question.

Question: Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into
[A].

pBR 322 based plasmid vector

[B].

pRK 2013

[C].

pRN 3

[D].

all of these

Answer: Option A

Explanation:

No answer description available for this question.

Question: In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by
[A].

vir A

[B].

vir B

[C].

vir C

[D].

vir D

Answer: Option D

Explanation:

No answer description available for this question.