Fluid Flow

Question: Bubble free aeration systems requires
[A].

silicone tubing or gas permeable membranes to facilitate gas transfer

[B].

carbon dioxide instead of air to supply oxygen

[C].

silicone tubing or gas permeable membranes to remove carbon dioxide by oxygen in the form of hydrogen peroxide

[D].

filled headspace of the reactor with carbon dioxide

Answer: Option A

Explanation:

No answer description available for this question.

Question: In animal cell bioreactor, bubble damage can be minimized by
[A].

the addition of a shear protectorant

[B].

using a bubble free oxygen delivery system

[C].

headspace aeration

[D].

all of the above

Answer: Option D

Explanation:

No answer description available for this question.

Question: Which of the following can act as a shear protectorant?
[A].

Serum

[B].

Silicone oil

[C].

Glucose

[D].

Glutamic acid

Answer: Option A

Explanation:

No answer description available for this question.

Question: Pluronic F68 is added to cell culture media as
[A].

a nutrient

[B].

a shear protectorant

[C].

to increase oxygen transfer rates

[D].

all of these

Answer: Option B

Explanation:

No answer description available for this question.

Question: The best described action of Pluronic F68 (non-ionic detergent) in a cell culture medium is that, it
[A].

acts on the surface properties of the cells and the culture medium

[B].

provides nutrients for the cells

[C].

acts as a flocculant

[D].

acts as a coagulant

Answer: Option A

Explanation:

No answer description available for this question.

Question: A liquid is flowing at 11,400 l.h-1 along a pipeline having a diameter of 4 cm. If the liquid has a density of 1 g.ml-1 and viscosity of 0.001kg.m-1.s-1, then the liquid would be in the
[A].

laminar phase

[B].

transient phase

[C].

turbulent phase

[D].

any of the phase

Answer: Option C

Explanation:

No answer description available for this question.

Question: Reynolds number for turbulent flow over a plate should be more than
[A].

5 x 10-5

[B].

106

[C].

5 x 105

[D].

500

Answer: Option B

Explanation:

No answer description available for this question.

Question: Reynolds number for laminar flow over a plate should be less than
[A].

500 mm

[B].

5 x 10-5

[C].

5 x 105

[D].

5 m

Answer: Option C

Explanation:

No answer description available for this question.

Question: Which of the following describes the detrimental effect of bubbles on animal cells?
[A].

Cells trapped on the surface of bubbles can be damaged when the bubbles explode, releasing their pressure and surface energies

[B].

Cells trapped in the moving foam can be dragged apart by the bubbles

[C].

Both (a) and (b)

[D].

Bubbles can explode in the liquid medium causing the release of ultraviolet radiation

Answer: Option C

Explanation:

No answer description available for this question.

Question: Filamentous fungal cells are more shear sensitive than bacterial cells because of
[A].

the presence of citin in cell walls of filamentous fungi

[B].

larger in size than bacterial cells and therefore are more susceptible to shear forces

[C].

fungal cells are much larger than bacterial cells

[D].

all of the above

Answer: Option D

Explanation:

No answer description available for this question.