Cloning Vectors

Question: Cosmid vectors are used for
[A].

cloning small fragments of DNA

[B].

cloning large fragments of DNA

[C].

cloning prokaryotic DNA only

[D].

cloning eukaryotic DNA only

Answer: Option B

Explanation:

No answer description available for this question.

Question: Cosmid vectors are
[A].

plasmids that contain fragment of λ DNA including the cos site

[B].

phages that lack cos site

[C].

plasmids that have no selection marker

[D].

cryptic plasmids

Answer: Option A

Explanation:

No answer description available for this question.

Question: Conjugative plasmids
[A].

exhibit antibiotic resistance

[B].

do not exhibit antibiotic resistance

[C].

carry transfer genes called the tra genes

[D].

do not carry transfer genes

Answer: Option C

Explanation:

No answer description available for this question.

Question: Cryptic plasmids
[A].

do not exhibit any phenotypic trait

[B].

exhibit many phenotypic traits

[C].

exhibit one phenotypic traits

[D].

exhibit antibiotic resistance

Answer: Option A

Explanation:

No answer description available for this question.

Question: Maximum size of foreign DNA that can be inserted into a replacement vector is
[A].

25-30 kb

[B].

18-20 kb

[C].

20-25 kb

[D].

40-50 kb

Answer: Option C

Explanation:

No answer description available for this question.

Question: Maximum size of foreign DNA that can be inserted into an insertion vector is
[A].

35 kb

[B].

18 kb

[C].

50 kb

[D].

27 kb

Answer: Option B

Explanation:

No answer description available for this question.

Question: Cos site of the cosmids
[A].

consists of 12 bases

[B].

helps whole genome in circularization and ligation

[C].

both (a) and (b)

[D].

contains cleavage site

Answer: Option C

Explanation:

No answer description available for this question.

Question: Cosmids lack
[A].

genes coding for viral proteins

[B].

origin of replication

[C].

marker genes coding for replication

[D].

cleavage site for the insertion of foreign DNA

Answer: Option A

Explanation:

No answer description available for this question.

Question: pBR 322 has/have which of the following selection marker(s)?
[A].

Ampr

[B].

Tetr

[C].

Both (a) and (b)

[D].

Kanr

Answer: Option C

Explanation:

No answer description available for this question.

Question: A plasmid can be considered as a suitable cloning vector if
[A].

it can be readily isolated from the cells

[B].

it possesses a single restriction site for one or more restriction enzymes

[C].

insertion of foreign DNA does not alter its replication properties

[D].

All of the above

Answer: Option D

Explanation:

No answer description available for this question.