Cloning Vectors

Question: P1 cloning vector allow cloning of DNA of the length of
[A].

100 kbp

[B].

50 kbp

[C].

20 kbp

[D].

10 kbp

Answer: Option A

Explanation:

No answer description available for this question.

Question: P1 cloning vector is the example of
[A].

plasmid

[B].

cosmid

[C].

bacteriophage

[D].

phagemid

Answer: Option C

Explanation:

No answer description available for this question.

Question: Charon 34 and Charon 35 can clone DNA upto
[A].

1-2 kb

[B].

6-7 kb

[C].

9-20 kb

[D].

30-35 kb

Answer: Option C

Explanation:

No answer description available for this question.

Question: Charon vectors are different from EMBL vectors because
[A].

they have more extensive range of restriction targets with in their polylinkers.

[B].

physical separation of lambda arm from central fragment is required

[C].

both (a) and (b)

[D].

physical separation of lambda arm from central fragment is not required

Answer: Option C

Explanation:

No answer description available for this question.

Question: Charon 34 and Charon 35 are the examples of
[A].

plasmid vector

[B].

cosmid vector

[C].

phage vector

[D].

phagemid vector

Answer: Option C

Explanation:

No answer description available for this question.

Question: EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to
[A].

6-7 kb

[B].

15-25 kb

[C].

40-44 kb

[D].

1-2 kb

Answer: Option B

Explanation:

No answer description available for this question.

Question: λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to
[A].

6-7 kb

[B].

1-2 kb

[C].

40-44 kb

[D].

20-23 kb

Answer: Option A

Explanation:

No answer description available for this question.

Question: Inserted DNA in λ gt 11 can be expressed as
[A].

β-galactosidase fused protein

[B].

free protein in the cytoplasm

[C].

free protein that is secreted out

[D].

all of the above

Answer: Option A

Explanation:

No answer description available for this question.

Question: Difference between λ gt 10 and λ gt 11 vectors is that
[A].

λ gt 11 is an expression vector

[B].

λ gt 10 is an expression vector

[C].

λ gt 10 is a replacement vector

[D].

λ gt 11 is a replacement vector

Answer: Option A

Explanation:

No answer description available for this question.

Question: M 13 is an example of
[A].

filamentous phage

[B].

single stranded DNA vector

[C].

both (a) and (b)

[D].

plasmid

Answer: Option C

Explanation:

No answer description available for this question.