Biotechnology

Question: Direct DNA uptake by protoplasts can be stimulated by
[A].

polyethylene glycol (PEG)

[B].

decanal

[C].

luciferin

[D].

all of these

Answer: Option A

Explanation:

No answer description available for this question.

Question: Intermediate vectors containing T-DNA are conjugation deficient. Thus conjugation is mediated in presence of which of the following plasmid?
[A].

pRK 2013

[B].

pRN 3

[C].

either (a) or (b)

[D].

none of these

Answer: Option C

Explanation:

No answer description available for this question.

Question: Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into
[A].

pBR 322 based plasmid vector

[B].

pRK 2013

[C].

pRN 3

[D].

all of these

Answer: Option A

Explanation:

No answer description available for this question.

Question: In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by
[A].

vir A

[B].

vir B

[C].

vir C

[D].

vir D

Answer: Option D

Explanation:

No answer description available for this question.

Question: Which of the following plant signal molecules regulate the expression of vir B, C, D and E in case of tobacco?
[A].

Acetosyringone

[B].

α-hydroxy syringone

[C].

Both (a) and (b)

[D].

None of these

Answer: Option C

Explanation:

No answer description available for this question.

Question: On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of
[A].

12 bp

[B].

20 bp

[C].

25 bp

[D].

30 bp

Answer: Option C

Explanation:

No answer description available for this question.

Question: Integrated octopine T DNA occurs as
[A].

single segment

[B].

two segments

[C].

three segments

[D].

four segments

Answer: Option B

Explanation:

No answer description available for this question.

Question: Integrated nopaline T-DNA occurs as
[A].

single segment

[B].

two segments

[C].

three segments

[D].

four segments

Answer: Option A

Explanation:

No answer description available for this question.

Question: In a plant tumour cell
[A].

complete Ti plasmid is incorporated in plant nuclear DNA

[B].

different parts of the Ti-plasmid are incorporated

[C].

only a small specific segment of callus T DNA is incorporated

[D].

may vary from plant to plant

Answer: Option C

Explanation:

No answer description available for this question.

Question: Opine synthesis is the property
[A].

conferred to plant cells when it transformed by Agrobacterium tumefaciens

[B].

determined by the bacteria Agrobacterium tumefaciens

[C].

both (a) and (b)

[D].

of normal plant cells

Answer: Option C

Explanation:

No answer description available for this question.