Biochemistry

Question: Automated DNA sequencing uses the chain termination method
[A].

but with an oligonucleotide primer labeled with a fluorescent dye

[B].

but with a polynucleotide primer labeled with a fluorescent dye

[C].

with no primer

[D].

either (b) or (c)

Answer: Option A

Explanation:

No answer description available for this question.

Question: DNA can be sequenced by the
[A].

chemical method

[B].

chain termination procedure

[C].

both (a) and (b)

[D].

physical method

Answer: Option C

Explanation:

No answer description available for this question.

Question: DNA polymerases I also has
[A].

5′-3′ exonuclease activity

[B].

3′-5’exonuclease activity

[C].

both (a) and (b)

[D].

5′-3′ endonuclease activity

Answer: Option B

Explanation:

No answer description available for this question.

Question: E.coli DNA polymerases II and III lack
[A].

5′ → 3′ exonuclease activity

[B].

5′ → 3′ endonuclease activity

[C].

partially 5′ → 3′ exonuclease activity

[D].

partially 5′ → 3′ endonuclease activity

Answer: Option A

Explanation:

No answer description available for this question.

Question: During conventional transformation experiments E. coli cells and plasmid DNA interacts in an environment of
[A].

high temperature and Ca++

[B].

low temperature and Ca++

[C].

high temperature and Mg++

[D].

low temperature and Mg++

Answer: Option B

Explanation:

No answer description available for this question.

Question: In sperm heads, DNA is particularly highly condensed and the histones are replaced with small basic protein called
[A].

protamines

[B].

purines

[C].

pyrimidines

[D].

all of the above

Answer: Option A

Explanation:

No answer description available for this question.

Question: Which of the following species lack a ‘classical’ histone H1?
[A].

Saccharomyces cerevisiae

[B].

Strongylocentrotus purpuratus

[C].

Xenopus laevis

[D].

Arabidopsis thaliana

Answer: Option A

Explanation:

No answer description available for this question.

Question: Nucleosome is
[A].

an octamer of four histones with H1 protein

[B].

an hexamer of three histones with H1 protein

[C].

an hexamer of three histones without H1 protein

[D].

an octamer of four histones without H1 protein

Answer: Option D

Explanation:

No answer description available for this question.

Question: In DNA double helix, the two DNA chains are held together by
[A].

covalent bonds between the pair of bases

[B].

hydrogen bonds between the pair of bases

[C].

ionic bonds between the pair of bases

[D].

none of the above

Answer: Option B

Explanation:

No answer description available for this question.

Question: In DNA, nucleotides are covalently joined together by
[A].

3′, 5′ phosphodiester bonds to form a repetitive sugar-phosphate chain

[B].

2′, 5′ phosphodiester bonds to form a repetitive sugar-phosphate chain

[C].

2′, 3′ phosphodiester bonds to form a repetitive sugar-phosphate chain

[D].

3′, 4′ phosphodiester bonds to form a repetitive sugar-phosphate chain

Answer: Option A

Explanation:

No answer description available for this question.