Biochemistry

Question: Column efficiency is measured in terms of number of plates which is
[A].

inversely related to the square of the peak width

[B].

directly related to the square of the peak width

[C].

inversely related to the cube root of the peak width

[D].

directly related to the square of the peak width

Answer: Option A

Explanation:

No answer description available for this question.

Question: For a typical adsorbent such as silica gel, the most popular pore diameters are
[A].

10 and 50 A°

[B].

60 and 100 A°

[C].

100 and 150 A°

[D].

150 and 200 A°

Answer: Option B

Explanation:

No answer description available for this question.

Question: Dwell volume is defined as
[A].

the volume of solvent contained in a liquid chromatographic column

[B].

the time required for the gradient to reach the column

[C].

the volume of the column between the point at which solvents are mixed and the beginning of the column

[D].

none of these

Answer: Option C

Explanation:

No answer description available for this question.

Question: An eluotropic series
[A].

ranks solvents by their relative abilities to displace solutes from a given absorbent

[B].

ranks column packing material by their relative abilities to retain solutes on the column

[C].

is a measure of the solvent adsorption energy

[D].

none of the above

Answer: Option A

Explanation:

No answer description available for this question.

Question: Which of the following statements is true for a refractive index detector in HPLC?
[A].

It is more sensitive than a UV detector

[B].

It can only be used for isocratic elutions

[C].

It does not respond to many solutes

[D].

none of above

Answer: Option C

Explanation:

No answer description available for this question.

Question: A gradient elution in HPLC is one in which the composition of the solvent
[A].

remains constant

[B].

is changed continuously or in a series of steps

[C].

both (a) and (b)

[D].

none of the above

Answer: Option B

Explanation:

No answer description available for this question.

Question: An isocratic elution in HPLC is one in which the composition of the solvent
[A].

remains constant

[B].

changes continuously

[C].

changes in a series of steps

[D].

none of these

Answer: Option A

Explanation:

No answer description available for this question.

Question: In reversed phase HPLC, there is a
[A].

non polar solvent/polar column

[B].

polar solvent/non-polar column

[C].

non polar solvent/non-polar column

[D].

any of the above

Answer: Option B

Explanation:

No answer description available for this question.