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Biochemistry

If the egg white protein, ovalbumin, is denatured in a hard-boiled egg, then which of the following is least affected?

Biochemistry, Protein Stability /
Question: If the egg white protein, ovalbumin, is denatured in a hard-boiled egg, then which of the following is least affected?
[A].

The primary structure of ovalbumin

[B].

The secondary structure of ovalbumin

[C].

The tertiary structure of ovalbumin

[D].

The quaternary structure of ovalbumin

Answer: Option A

Explanation:

No answer description available for this question.

If the egg white protein, ovalbumin, is denatured in a hard-boiled egg, then which of the following is least affected? Read More »

Biochemistry, Protein Stability

Buried hydrophobic side chains in a globular protein fit into a hole formed by the side chains of

Biochemistry, Protein Stability /
Question: Buried hydrophobic side chains in a globular protein fit into a hole formed by the side chains of
[A].

1-3 other amino acids

[B].

5-7 other amino acids

[C].

9-12 other amino acids

[D].

13-15 other amino acids

Answer: Option B

Explanation:

No answer description available for this question.

Buried hydrophobic side chains in a globular protein fit into a hole formed by the side chains of Read More »

Biochemistry, Protein Stability

Gel-filtration chromatography separates on the basis of

Biochemistry, Protein Purification /
Question: Gel-filtration chromatography separates on the basis of
[A].

size and shape using porous beads packed in a column

[B].

size using porous beads packed in a column

[C].

shape using porous beads packed in a column

[D].

none of the above

Answer: Option A

Explanation:

No answer description available for this question.

Gel-filtration chromatography separates on the basis of Read More »

Biochemistry, Protein Purification

During successful purification scheme, this may be expected that the

Biochemistry, Protein Purification /
Question: During successful purification scheme, this may be expected that the
[A].

specific activity increases

[B].

specific activity decreases

[C].

number of proteins in the sample decreases

[D].

both (a) and (c)

Answer: Option D

Explanation:

No answer description available for this question.

During successful purification scheme, this may be expected that the Read More »

Biochemistry, Protein Purification

A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?

Biochemistry, Protein Purification /
Question: A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?
[A].

1,000 units/mg

[B].

10,000 units/mg.

[C].

100,000 units/mg

[D].

1,000,000 units/mg

Answer: Option C

Explanation:

No answer description available for this question.

A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample? Read More »

Biochemistry, Protein Purification

The best way to determine the location of protein in the purification scheme is to measure the

Biochemistry, Protein Purification /
Question: The best way to determine the location of protein in the purification scheme is to measure the
[A].

rate of ATP synthesis

[B].

changes in the refractive index

[C].

UV absorption

[D].

mass spectroscopy of the protein

Answer: Option A

Explanation:

No answer description available for this question.

The best way to determine the location of protein in the purification scheme is to measure the Read More »

Biochemistry, Protein Purification

Cleavage of DNA by EcoRI endonuclease results in the formation of

Biochemistry, Protein And Nucleic Acid Interactions /
Question: Cleavage of DNA by EcoRI endonuclease results in the formation of
[A].

two 3′-OH ends and two 5′-phosphate ends

[B].

one 3′-OH end and one 5′-phosphate end

[C].

two 3′-phosphate ends and two 3′-OH ends

[D].

one 3′-OH end and two 5′-phosphate end

Answer: Option A

Explanation:

No answer description available for this question.

Cleavage of DNA by EcoRI endonuclease results in the formation of Read More »

Biochemistry, Protein And Nucleic Acid Interactions