Biochemical Engineering

Question: Which of the following is considered as a disadvantage to the adsorption method of immobilization?
[A].

It is possible to separate and purify the enzymes while being immobilized

[B].

The enzymes are not usually deactivated by adsorption

[C].

The adsorption is a reversible process

[D].

State of immobilization is very sensitive to solution pH, ionic strength and temperature

Answer: Option D

Explanation:

No answer description available for this question.

Question: Which of the following is the commonly employed adsorbents?
[A].

Calcium carbonate

[B].

Alumina

[C].

Celluloses

[D].

All of these

Answer: Option D

Explanation:

No answer description available for this question.

Question: Which of the following is not a physical method of immobilization?
[A].

Adsorption

[B].

Entrapment

[C].

Micro encapsulation

[D].

None of these

Answer: Option D

Explanation:

No answer description available for this question.

Question: Water insoluble enzymes can be prepared by using multifunctional agents that are bifunctional in nature and have
[A].

low molecular weight

[B].

high molecular weight

[C].

high equivalent weight

[D].

low reactivity

Answer: Option A

Explanation:

No answer description available for this question.

Question: Commonly employed water insoluble supports for the covalent attachment of enzyme include
[A].

acrylamide based polymers

[B].

polypeptides

[C].

dextran

[D].

all of these

Answer: Option D

Explanation:

No answer description available for this question.

Question: The immobilization technique involving physical method is
[A].

covalent bond formation dependent

[B].

non-covalent bond formation dependent

[C].

both (a) and (b)

[D].

ionic bond formation dependent

Answer: Option B

Explanation:

No answer description available for this question.

Question: The immobilized technique involving chemical method is
[A].

covalent bond formation dependent

[B].

non-covalent bond formation dependent

[C].

both (a) and (b)

[D].

ionic bond formation dependent

Answer: Option A

Explanation:

No answer description available for this question.

Question: The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by
[A].

copolymerization or microencapsulation

[B].

crosslinking using multifunctional reagents

[C].

adsorption

[D].

all of the above

Answer: Option A

Explanation:

No answer description available for this question.

Question: Damkohler number(NDa) is
[A].

the ratio of the maximum reaction rate to the maximum mass transfer rate

[B].

the ratio of the minimum reaction rate to the maximum mass transfer rate

[C].

the ratio of the maximum reaction rate to the minimum mass transfer rate

[D].

the ratio of the minimum reaction rate to the minimum mass transfer rate

Answer: Option A

Explanation:

No answer description available for this question.

Question: Which is not the method for producing immobilized enzymes with multifunctional reagents?
[A].

Enzymes are adsorbed on the surface active support followed by intermolecular cross linking

[B].

Functional groups are introduced on the support to react co-valently with enzymes

[C].

Enzymes are cross linked intermolecularly

[D].

None of the above

Answer: Option D

Explanation:

No answer description available for this question.